BID Antibody (Human Specific)

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9). 1.Wang, K. et al. (1996) Genes Dev 10, 2859-69. 2.Luo, X. et al. (1998) Cell 94, 481-90. 3.Li, H. et al. (1998) Cell 94, 491-501. 4.Gross, A. et al. (1999) J Biol Chem 274, 1156-63. 5.Li, P. et al. (1997) Cell 91, 479-89. 6.Zou, H. et al. (1999) J Biol Chem 274, 11549-56. 7.Saleh, A. et al. (1999) J Biol Chem 274, 17941-5. 8.Yin, X.M. et al. (1999) Nature 400, 886-91. 9.Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.