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Biotin Labeling for Avitag Protein Kit
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对比
咨询Accession: P06709;
内毒素水平: <0.5EU/ug;
形态: 20mM Bicine, 50% Glycerol, pH8.3;
分子量: 35 kDa (Reducing);
纯度: ＞95% by SDS-PAGE and HPLC;
来源: E.coli；
序列: <p>Met1-Lys321</p>
<p>MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK.</p>
Component:
| Items | Compositon | Amount | |
| BirA | 20mM Bicine, 50%Glycerol, pH8.3 | 200ug | 1mg |
| 10×Buffer A | 500mM Bicine, pH8.3 | 2.5mL | 12.5mL |
| 10×Buffer B | 100mM ATP, 100mM MgOAc, 500uM D-Biotin | 2.5mL | 12.5mL |
| 10×Buffer C | 500uM D-Biotin | 2.5mL | 12.5mL |
参考图片
Note: 10nmol Avi-tagged protein substrate were labeled by 2μg BirA at 37℃ for 30min. All sample lanes are loaded 4μg protein, respectively. Streptavidin has 4 biotin-binding sites, and may associate with 1 to 4 chains of the biotinylated protein. Therefore, the extent of protein biotinylation can be calculated based on the ratio of biotinylated protein to original substrate protein.
2μg（N: non-reducing condition, R: reducing condition).