Bovine Early Growth Response Protein 4 ELISA Kit

early growth response protein 4

Intended Uses: This EGRP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine EGRP4. This ELISA kit for research use only, not for therapeutic or test applications! Principle of the Assay: EGRP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EGRP4 antibody and an EGRP4-HRP conjugate. The assay sample and buffer are incubated together with EGRP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGRP4 concentration since EGRP4 from samples and EGRP4-HRP conjugate compete for the anti-EGRP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by EGRP4 from the sample, fewer sites are left to bind EGRP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGRP4 concentration in each sample is interpolated from this standard curve.

Bovine

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

0.1 ng/mL

1961

Q05215 [Other Products]