Bovine Heat Shock 70kDa Protein 4 ELISA Kit

heat shock 70 kDa protein 4

Principle of the assay: HSPA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSPA4 antibody and an HSPA4-HRP conjugate. The assay sample and buffer are incubated together with HSPA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPA4 concentration since HSPA4 from samples and HSPA4-HRP conjugate compete for the anti-HSPA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPA4 from the sample, fewer sites are left to bind HSPA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPA4 concentration in each sample is interpolated from this standard curve.

Bovine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1 ng/mL.

3308

P34932 [Other Products]