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Bovine Trypsin ELISA Kit
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产品介绍
产品介绍
产品信息
抗原名称
trypsin
商品描述
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with trypsin. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for trypsin. The competitive inhibition reaction is launched between with pre-coated trypsin and trypsin in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of trypsin in the samples. The color development is stopped and the intensity of the color is measured.
组成成分
微量ELISA板（涂覆胰蛋白酶96孔板）；标准品（重组胰蛋白酶）；HRP结合物100X浓缩 （辣根过氧化物酶标记的抗牛胰蛋白酶抗体）；HRP结合物稀释剂（磷酸盐缓冲液，HRP链霉亲和素）；样品稀释缓冲液（磷酸盐缓冲液）；洗涤缓冲液25X（0.05%吐温20、99.95%水）；底物基质（四甲基联苯胺溶液）；终止液（20%氢氧化钠溶液）
应用
反应种属
Bovine
检测范围
62.5 pg/ml-4000 pg/ml
检测类型
Quantitative Competitive
样本类型以及上样量
Serum, plasma, tissue homogenates
敏感性
< 15.6 pg/ml
背景
别名
Bovine Trypsin ELISA Kit; Trypsin
制备和贮存
保存方式
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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