PathScan ® RP BRD9 Sandwich ELISA Kit

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1, to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).GLTSCR1 and its paralog GLTSCR1L along with BRD9 have been identified as unique subunits of the non-canonical BAF (ncBAF) or GBAF complex. This complex contains either GLTSCR1 or GLTSCR1L instead of an ARID subunit, while also lacking the BAF45, BAF47, and BAF57 subunits. GBAF maps to regions distinct from the PBAF and canonical BAF complexes and has also been shown to be a synthetic lethal target for BAF driven cancers such as synovial sarcomas and rhabdoid tumors (10-12). 1.Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 2.Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73. 3.Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11. 4.Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81. 5.Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17. 6.Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32. 7.Morettini, S. et al. (2008) Front Biosci 13, 5522-32. 8.Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6. 9.Simone, C. (2006) J Cell Physiol 207, 309-14. 10.Alpsoy, A. and Dykhuizen, E.C. (2018) J Biol Chem 293, 3892-3903. 11.Mashtalir, N. et al. (2018) Cell 175, 1272-1288.e20. 12.Michel, B.C. et al. (2018) Nat Cell Biol 20, 1410-1420.