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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
BrdU
来源纯化
Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA.
宿主
Mouse
简单描述
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated BrdU (Bu20a) Mouse mAb #5292.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
同种型
Mouse IgG1
克隆号
Bu20a
应用
反应种属
All
目标/特异性
Specificity/Sensitivity
BrdU (Bu20a) Mouse mAb (PE Conjugate) detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Species Reactivity:
All Species Expected
敏感性
Endogenous
背景
背景
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4).
1.Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7.
2.Leif, R.C. et al. (2004) Cytometry A 58, 45-52.
3.Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44.
4.Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
参考图片
Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 min), using BrdU (Bu20a) Mouse mAb (PE conjugate) versus DRAQ5® (DNA content).
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