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Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4). 1.Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7. 2.Leif, R.C. et al. (2004) Cytometry A 58, 45-52. 3.Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44. 4.Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.
参考图片
Immunohistochemical analysis of paraffin-embedded HeLa cells, without (left) and with (right) BrdU incorporation, using BrdU (Bu20a) Mouse mAb.免疫组织化学方法检测石蜡包埋Hela细胞，不伴（左侧）或伴有Brdu掺入（右侧），使用的抗体为 BrdU (Bu20a) Mouse mAb。
Confocal immunofluorescent analysis of HeLa cells using BrdU (Bu20a) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测Hela细胞，是由的抗体为BrdU (Bu20a) Mouse mAb ，呈绿色。蓝色伪彩为DNA荧光染料，产品信息为 DRAQ5® #4084 。
Flow cytometric analysis of Jurkat cells, untreated (blue) or BrdU incorporated for 30 minutes (green), using BrdU (Bu20a) Mouse mAb.流式细胞术检测Jurkat细胞，不伴有（蓝色）或伴有Brdu掺入（绿色），使用的抗体为BrdU (Bu20a) Mouse mAb。
Flow cytometric analysis of Jurkat cells incorporated with BrdU (30 minutes), using BrdU (Bu20a) Mouse mAb versus propidium iodide (DNA content).流式细胞术检测Jurkat细胞，伴有Brdu掺入（30分钟），使用的抗体为BrdU (Bu20a) Mouse mAb，碘化乙啶检测DNA含量作为对照。