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- 描述数量/尺寸零件号EntrezGene ID
- PE Mouse IgG1 κ Isotype Control100 Tests (1 ea)51-35405X-2N/A
- PE Mouse Anti-BrdU100 Tests (1 ea)51-33285XN/A
参考图片
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System
Two-color flow cytometric analysis of DNA synthesis by REH cells. REH (Human leukemia, ATCC CRL-8286) cells were labeled with 1mM BrdU for one hour, washed, fixed with 75% ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 κ Isotype Control (Left Panel) or PE Mouse Anti-BrdU antibody (Right Panel; Cat No. 556029) followed by staining with DAPI Solution (Cat. No. 564907; nucleic acid stain). Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (total cellular DNA) versus incorporated BrdU (representing newly-synthesized DNA) were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II System