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Target: Goat
Host: Donkey
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat Serum ProteinsConjugate: Brilliant Violet 421™
Product Category: Brilliant Violet™ Conjugates for Multiple Labeling
Clonality: Polyclonal
RRID: AB_2651102
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Note: Store stained specimens at 4°C to preserve fluorescence.
Buffer: 0.01M Sodium Phosphate, 0.15M NaCl, 0.005% Tween 20, pH 7.2
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
1:50 - 1:200 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Brilliant Violet 421™
Amax: 407 Emax: 421nmBD Brilliant™ Violet 421 (BV421) has an Excitation peak at 407 nm with maximal emission at 421 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
5-color imaging is possible with BV421, in combination with BV480, Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor® 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.