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Bst DNA Polymerase, Full length

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Bst DNA Polymerase, Full length

品牌： UA BIOSCIENCE

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参数

分子量：

纯度：

产品介绍

产品信息

分子量

100kDa (Reducing)

生物活性

5U/μl

酶活定义

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C

纯度

＞95% by SDS-PAGE

表达系统

E.coli

缓冲体系

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C

组合货号

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、100 mM KCl、20mM MgSO4、0.1% Tween® 20、pH 8.8@25°C
Magnesium Sulfate (MgSO4) Solution：100mM MgSO4

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电泳JSON

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2μg (R: reducing condition, N: non-reducing condition).

"}]

背景

别名

DNA polymerase I

背景

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity. It can be used to isothermal DNA amplification and Primer extension.

制备和贮存

保存方式

Store at -25 ~ -15℃ for 2 years

文献

1. Genetic Analysis: Biomolecular Engineering, 1996, 12(5-6):185-195.
2. BioTechniques, 1991, 11(1):76-8, 80, 82-7.

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货号：

UA070064-500U

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