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Bst DNA Polymerase, Large fragment

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Bst DNA Polymerase, Large fragment

品牌： UA BIOSCIENCE

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参数

分子量：

纯度：

产品介绍

产品信息

分子量

67kDa (Reducing)

生物活性

8U/μl

酶活定义

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C

纯度

＞95% by SDS-PAGE

表达系统

E.coli

缓冲体系

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C

组合货号

50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C
10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、20mM MgSO4、100 mM KCl、1% Tween® 20、pH 8.8@25°C
Magnesium Sulfate (MgSO4) Solution：100mM MgSO4

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电泳JSON

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2μg (R: reducing condition, N: non-reducing condition).

"}]

背景

别名

DNA polymerase I

背景

Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the
5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity. It can be use to isothermal amplification (LAMP), DNA sequencing through high GC regions and Rapid Sequencing from nanogram amounts of DNA template.

制备和贮存

保存方式

Store at -25 ~ -15℃ for 2 years

文献

1. Nucleic Acids Research, 2000, 28(12):E63.
2. Chemical Communications, 2014, 50(28):3747-3749.
3. Current Protocols in Molecular Biology, 2014(Suppl.105):15.14.1-15.14.14.

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货号：

UA070065-800U

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800U

8000U

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