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c-Rel (E8Z5Y) XP ® Rabbit mAb

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c-Rel (E8Z5Y) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human c-Rel protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Immunocytochemistry)	1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Rabbit IgG

分子量

应用

目标/特异性

Specificity/Sensitivity

c-Rel (E8Z5Y) XP Rabbit mAb recognizes endogenous levels of total c-Rel protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).c-Rel contains an amino-terminal DNA-binding domain, referred to as the REL homology domain (REH), and carboxy-terminal transactivation domains. The c-Rel protein is typically inhibited in unstimulated cells by IκBα and IκBβ. c-Rel expression is highest in hematopoietic cells with extensive research studies demonstrating its role in immune cell function and pathogenesis of disease (12,13). 1.Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2.Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3.Haskill, S. et al. (1991) Cell 65, 1281-9. 4.Thompson, J.E. et al. (1995) Cell 80, 573-82. 5.Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6.Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7.Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8.Chen, Z.J. et al. (1996) Cell 84, 853-62. 9.Senftleben, U. et al. (2001) Science 293, 1495-9. 10.Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11.Xiao, G. et al. (2001) Mol Cell 7, 401-9. 12.Gilmore, T.D. and Gerondakis, S. (2011) Genes Cancer 2, 695-711. 13.Fullard, N. et al. (2012) Int J Biochem Cell Biol 44, 851-60.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #57603.

数据库链接

Entrez-Gene ID

5966

UniProt ID

Q04864

参考图片

Flow cytometric analysis of HL-60 cells (blue, low-expressing) and RT-4 cells (green, high-expressing) using c-Rel (E8Z5Y) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using c-Rel (E8Z5Y) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Lower expression of c-Rel protein in HL-60, MDA-MB-453, and Caki-1 cells is consistent with the predicted expression pattern.

Immunoprecipitation of c-Rel protein from RT-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is c-Rel (E8Z5Y) XP® Rabbit mAb. Western blot analysis was performed using c-Rel (E8Z5Y) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human kidney using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Ri-1 cell pellet (left, high-expressing) or MDA-MB-453 cell pellet (right, low-expressing) using c-Rel (E8Z5Y) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human lymph node using c-Rel (E8Z5Y) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Confocal immunofluorescent analysis of RT-4 cells (high-expressing), either untreated (left) or treated with hTNF-α (20 ng/mL, 30 min; middle), and Caki-1 cells (right, low-expressing) using c-Rel (E8Z5Y) XP® Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue).

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货号：

82000T

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