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- 描述数量/尺寸零件号EntrezGene ID
- Capture Antibody Purified anti-Human C5b-9N/A51-9004080N/A
- Human C5b-9 Lyophilized StandardN/A51-9004086N/A
- Detection Antibody Biotin anti-Human C5b-9N/A51-9004167N/A
- Streptavidin-Horseradish Peroxidase conjugate (SAv-HRP) 250XN/A51-9005408N/A
参考图片
Representative standard curve using the BD OptEIA Human C5b-9 ELISA Set. A standard curve should be ran for each assay.
This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.
Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (30 ng/mL), then adding 100 µL of the 30 ng/mL standard to both that well and the 15 ng/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 15 ng/mL standard to the 7.5 ng/mL well. Continue these dilutions to the 0.469 ng/mL standard well, out of which the extra 100 µL should be discarded .
Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (30 ng/mL), then adding 100 µL of the 30 ng/mL standard to both that well and the 15 ng/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 15 ng/mL standard to the 7.5 ng/mL well. Continue these dilutions to the 0.469 ng/mL standard well, out of which the extra 100 µL should be discarded.