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hE-Cadherin Qkit (1 Kit)
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品牌： R&D Systems
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Product Summary
The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.
Sample Values
| Sample Type | Mean (ng/mL) | Range (ng/mL) |
| Serum (n=30) | 250 | 127-492 |
| EDTA Plasma (n=30) | 242 | 131-479 |
| Heparin Plasma (n=30) | 236 | 116-479 |
| Urine (n=10) | 60.6 | 3.03-121 |
| Saliva (n=10) | 145 | 21.4-278 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.42 | 7.06 | 14.6 | 2.19 | 6.55 | 14.2 |
| Standard Deviation | 0.047 | 0.145 | 0.266 | 0.136 | 0.368 | 0.845 |
| CV% | 1.9 | 2.1 | 1.8 | 6.2 | 5.6 | 6 |
Recovery
The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=30) | 242 | 131-479 |
| Heparin Plasma (n=30) | 236 | 116-479 |
| Saliva (n=10) | 145 | 21.4-278 |
| Serum (n=30) | 250 | 127-492 |
| Urine (n=10) | 60 | 3.03-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human E-Cadherin Quantikine ELISA Kit Summary
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Sensitivity
0.126 ng/mL
Assay Range
0.6 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human E-Cadherin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
背景
别名
Arc-1,CAD1,cadherin 1, E-cadherin (epithelial),cadherin 1, type 1, E-cadherin (epithelial),Cadherin-1,calcium-dependent adhesion protein, epithelial,CAM 120/80,CD324 antigen,CD324,CDH1,CDHE,cell-CAM 120/80,Cell-CAM120/80,ECAD,ECadherin,E-Cadherin,Epithelial cadherin,LCAM,L-CAM,UVOE-Cadherin,Uvomorulin
背景
Background: E-Cadherin
The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.
Entrez Gene IDs:
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names:
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
研究领域
制备和贮存
保存方式
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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