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N-Cadherin (D4R1H) XP ® Rabbit mAb

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N-Cadherin (D4R1H) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

N-Cadherin

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg526 of human N-cadherin protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
IHC Leica Bond	1:25 - 1:100
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Frozen)	1:400
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600

同种型

Rabbit IgG

分子量

140

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,

目标/特异性

Specificity/Sensitivity

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). 1.Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. 2.Christofori, G. (2003) EMBO J 22, 2318-23. 3.Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. 4.Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34. 5.Rabascio, C. et al. (2004) Cancer Res 64, 4373-7. 6.Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7. 7.Patel, I.S. et al. (2003) Int J Cancer 106, 172-7. 8.Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #84117.

数据库链接

Entrez-Gene ID

1000

UniProt ID

P19022

参考图片

Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Simple Western™ analysis of lysates (0.1 mg/mL) from A172 lysate using N-Cadherin (D4R1H) XP® Rabbit mAb #13116. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin Lymphoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.

Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with N-Cadherin (D4R1H) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of fixed frozen mouse choroid plexus labeled with N-Cadherin (D4R1H) XP® Rabbit mAb (green) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of fixed frozen mouse heart labeled with N-Cadherin (D4R1H) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

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货号：

13116S

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