老带新
hN-Cadherin MAb (Cl (25 ug)

hN-Cadherin MAb (Cl (25 ug)

分享
品牌: R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
来源宿主:
Mouse IgM
实验应用:
Flow Cytometry(2.5 µg/106 cells)
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
ACOGS, ARVD14, cadherin 2, type 1, N-cadherin (neuronal), Cadherin-2, calcium-dependent adhesion pro
宿主
Mouse IgM
免疫原
Mouse myeloma cell line NS0-derived recombinant human N-Cadherin
Ser26-Arg159
Accession # P19022
克隆号
691701

Scientific Data

Flow Cytometry Detection of N-Cadherin antibody in HeLa Human Cell Line antibody by Flow Cytometry.View Larger

Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13882, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

Human N-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human N-Cadherin in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse N-Cadherin is observed and no cross-reactivity with recombinant human (rh) E-Cadherin or rhCadherin-4/R-Cadherin is observed.
Source
Monoclonal Mouse IgM Clone # 691701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Cadherin
Ser26-Arg159
Accession # P19022
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

组成成分
IGG-免疫球蛋白G型抗体
应用
实验应用
Flow Cytometry(2.5 µg/106 cells)
背景
别名
ACOGS,ARVD14,cadherin 2, type 1, N-cadherin (neuronal),Cadherin-2,calcium-dependent adhesion protein, neuronal,CD325 antigen,CD325,CDH2,CDHN,CDw325,NCAD,N-cadherin 1,NCadherin,N-Cadherin,Neural cadherin,neural-cadherin,Neural Cadherin
背景

Background: N-Cadherin

N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130‑135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15‑20 kDa prodomain (aa 26‑159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N‑Cadherin proregion.

Long Name
Neural Cadherin
Entrez Gene IDs
1000 (Human); 12558 (Mouse); 83501 (Rat)
Alternate Names
ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325 antigen; CD325; CDH2; CDHN; CDw325; NCAD; N-cadherin 1; NCadherin; N-Cadherin; Neural cadherin; neural-cadherin
制备和贮存
溶解方法
Sterile PBS to a final concentration of 0.5 mg/mL.
保存方式

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13882, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
MAB13882-SP
一键复制
询价
25ug
100ug
选择数量
当前规格1件起购 
配送至
预计2-3周送达,快递: 免运费,若需干冰额外收费