Mouse Monoclonal Carbonic Anhydrase IX/CA9 Antibody (SPM487)

Protein A or G purified

Mouse

Microsomal fraction of human renal cortical tissue homogenate

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34411) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

200 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

0.2 mg/ml

0.05% Sodium Azide

Monoclonal

CA9

Flow Cytometry 1-2 ug/million cellsImmunocytochemistry/ Immunofluorescence 1-2 ug/mlImmunohistochemistry Immunohistochemistry-Paraffin 1-2 ug/ml

Hu, EqSpecies Glossary

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This monoclonal antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.