首页 抗体 一抗 一抗 Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb
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产品介绍
产品信息
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr287 of human CamKII-β protein.
商品描述
Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
应用
目标/特异性
Specificity/Sensitivity
Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb recognizes endogenous levels of CamKII-α protein only when phosphorylated at Thr286. This antibody also recognizes endogenous levels of CamKII-β and CamKII-γ protein only when phosphorylated at Thr287.
Species Reactivity:
Human, Mouse, Rat
背景
背景
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).
1.Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
2.Barria, A. et al. (1997) Science 276, 2042-5.
3.Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.
4.Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
UniProt ID
Q13554,Q13557,Q13555,Q9UQM7
参考图片
Western blot analysis of extracts from 293T cells, untransfected or transfected with specific CamKII proteins as indicated, using Phospho-CamKII (Thr286) (D21E4) Rabbit mAb. Antibody phosphospecificity was verified by preincubating the antibody in the absence of a peptide (-) or with either CamKII-β (Thr287) phosphopeptide (+) or CamKII-β (Thr287) non-phosphopeptide (+) prior to incubating the membrane.
Western blot analysis of extracts from MKN-45 cells treated with λ-phosphatase or Forskolin #3828 (30 μM, 20 min) using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb (upper) and CaMKII-α (D10C11) Rabbit mAb #11945 (lower).
Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb.
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