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cAMP Assay Kit (1 Kit)
品牌： R&D Systems
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Product Summary
The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.
Recovery
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 86-119 |
| Cell Lysis Buffer (n=1) | 91 | 87-94 |
| EDTA Plasma (n=4) | 105 | 92-120 |
| Heparin Plasma (n=4) | 98 | 85-111 |
| Saliva (n=4) | 97 | 80-117 |
| Serum (n=4) | 103 | 97-114 |
| Urine (n=6) | 100 | 91-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
cAMP Parameter Assay Kit Summary
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (10 uL)
Sensitivity
8.57 pmol/mL
Assay Range
3.8 - 240 pmol/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Measures cyclic AMP levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
背景
别名
cAMP,Cyclic AMP,Cyclic AMP
背景
Background: cAMP
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
Long Name:
Cyclic AMP
Alternate Names:
cAMP; Cyclic AMP
研究领域
制备和贮存
保存方式
Preparation and Storage
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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