Canine Anti-centriole and centrosome antibody IgA ELISA Kit

Principle of the Assay: ACA-IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACA-IgA antibody and an ACA-IgA-HRP conjugate. The assay sample and buffer are incubated together with ACA-IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACA-IgA concentration since ACA-IgA from samples and ACA-IgA-HRP conjugate compete for the anti-ACA-IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by ACA-IgA from the sample, fewer sites are left to bind ACA-IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACA-IgA concentration in each sample is interpolated from this standard curve.

Canine

50-1000ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL