Canine Cholecystokinin ELISA Kit

cholecystokinin

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CCK concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CCK antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CCK and CCK antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine

15.6pg/ml-500pg/ml.

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

2.0pg/ml.

885

P06307 [Other Products]