Canine Distal less Homeobox 5 ELISA Kit

distal-less homeobox 5

Principle of the Assay: DLX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DLX5 antibody and an DLX5-HRP conjugate. The assay sample and buffer are incubated together with DLX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DLX5 concentration since DLX5 from samples and DLX5-HRP conjugate compete for the anti-DLX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by DLX5 from the sample, fewer sites are left to bind DLX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DLX5 concentration in each sample is interpolated from this standard curve.

Canine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1749

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