Canine Macrophage stimulating protein ELISA Kit

macrophage-stimulating protein

Principle of the assay: MSP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MSP antibody and an MSP-HRP conjugate. The assay sample and buffer are incubated together with MSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSP concentration since MSP from samples and MSP-HRP conjugate compete for the anti-MSP antibody binding site. Since the number of sites is limited, as more sites are occupied by MSP from the sample, fewer sites are left to bind MSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSP concentration in each sample is interpolated from this standard curve.

Canine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0 pg/mL.

4485

P26927 [Other Products]