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Caspase-3 (D3R6Y) Rabbit mAb
品牌： CST
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对比
咨询分子量：
35, 19, 17
反应种属：
Human,Mouse,Rat,Monkey,
产品介绍
产品信息
抗原名称
Caspase-3
来源纯化
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-3 protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying Casp3. Cited in 374 publications. Validated for WB, WB, IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-3 (D3R6Y) Rabbit Monoclonal Antibody (CST #14220) is ready to ship.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
分子量
35, 19, 17
内毒素水平
猪
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Pig
目标/特异性
Specificity/Sensitivity
Caspase-3 (D3R6Y) Rabbit mAb recognizes endogenous levels of total caspase-3 protein. This antibody detects full-length caspase-3 as well as the large subunit (p20) of caspase-3 resulting from cleavage during apoptosis.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
1.Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2.Nicholson, D.W. et al. (1995) Nature 376, 37-43.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
836
UniProt ID
P42574
参考图片
Western blot analysis of various cell lines, untreated (-) or treated with Staurosporine #9953 (1 μM; 3 hr) or with Etoposide #2200 (25 μM, overnight), using Caspase-3 (D3R6Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MCF7 cells are negative for caspase-3 expression.
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