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Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
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Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb

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分子量:
17, 19
反应种属:
Human,Mouse,Rat,Monkey,
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Cleaved Caspase-3
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunoprecipitation1:50
Immunohistochemistry (Paraffin)1:2000
Immunofluorescence (Immunocytochemistry)1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)1:6400
同种型
Rabbit IgG
分子量
17, 19
内毒素水平
牛 , 犬 , 猪
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Bovine,Dog,Pig,
目标/特异性

Specificity/Sensitivity

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytoplasmic background may be observed in human and monkey samples.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). 1.Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 2.Nicholson, D.W. et al. (1995) Nature 376, 37-43.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
cleaved
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #94530.

数据库链接
Entrez-Gene ID
836
UniProt ID
P42574

参考图片

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).

Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine #9953 (1uM, 3hrs) or etoposide #2200 (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664. The virtual lane view (left) shows the target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunoprecipitation of Cleaved Caspase-3 from Jurkat cells treated with etoposide (25uM, 5hrs). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).

Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide® Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).

Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号:
9664S
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