全部商品分类
Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb
品牌： CST
下载产品说明书 下载COA 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询分子量：
18, 41, 43
反应种属：
Human
产品介绍
产品信息
荧光素标记
抗原名称
Cleaved Caspase-8
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp374 of human Caspase-8.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
同种型
Rabbit IgG
分子量
18, 41, 43
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human
目标/特异性
Specificity/Sensitivity
Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp374. This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone. A band of unknown origin is detected at 25 kDa in some cell lines.
Species Reactivity:
Human
敏感性
Endogenous
背景
背景
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
1.Muzio, M. et al. (1996) Cell 85, 817-27.
2.Boldin, M.P. et al. (1996) Cell 85, 803-15.
3.Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
cleaved
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
841
UniProt ID
Q14790
参考图片
Western blot analysis of HeLa and THP-1 cell extracts, untreated (-) or treated with cycloheximide (Chx) #2112 (10 μg/mL, 16 hr) followed by human TNFα (TNFα) #8902 (20 ng/mL, 4hr) using #9496 Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb (left), or #9746 Caspase-8 (1C12) Mouse mAb (right). A band of unknown origin is detected at 25 kDa in some cell lines.
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。