Caspase-9 (C9) Mouse mAb

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). 1.Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2.Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3.Liu, X. et al. (1996) Cell 86, 147-157. 4.Li, P. et al. (1997) Cell 91, 479-489. 5.Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6.Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7.Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8.Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9.Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10.MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.