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Non-p (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) R mAb
品牌： CST
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对比
咨询分子量：
92
来源宿主：
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein.
宿主
Rabbit
商品描述
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
| CUT&RUN | 1:100 |
同种型
Rabbit IgG
分子量
92
应用
目标/特异性
Specificity/Sensitivity
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and/or the canonical Wnt signaling pathway.
1.Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
2.Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3.Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4.Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5.Liu, C. et al. (2002) Cell 108, 837-47.
6.Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7.Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8.Morin, P.J. et al. (1997) Science 275, 1787-90.
研究领域
癌症,发育生物学与干细胞研究,纤维化,神经科学,
翻译后修饰
non-phosphate
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #33893.
数据库链接
Entrez-Gene ID
1499
UniProt ID
P35222
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