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Kit, mMCP-1 Qkit (1 Kit)
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对比
咨询Precision
Cell Culture Supernates, Tissue Lysates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 36.7 | 51.8 | 149 | 37.8 | 60 | 155 |
| Standard Deviation | 1.45 | 1.4 | 3.66 | 2.85 | 4.37 | 7.86 |
| CV% | 4 | 2.7 | 2.5 | 7.5 | 7.3 | 5.1 |
Recovery
The recovery of mouse MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 95-112 |
| Cell Lysis Buffer (n=4) | 88 | 79-101 |
| EDTA Plasma (n=4) | 84 | 75-93 |
| Heparin Plasma (n=4) | 93 | 80-101 |
| Serum (n=4) | 91 | 83-100 |
Linearity
Scientific Data
Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Summary
Background: CCL2/JE/MCP-1
CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.