BD Horizon™ BV421 Mouse Anti-Human CD197 (CCR7)

CD197

Mouse IgG2a

Human CCR7 Transfected Cell Line

The monoclonal antibody 150503 specifically binds to the human CC chemokine receptor, CCR7, also known as CD197. CCR7 (previously known as BLR2, EBI1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3β/Exodus 3/ELC/ CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL21. CCR7 mRNA is expressed mainly in lymphoid tissues including the spleen, lymph nodes and tonsil. CD197/CCR7 is expressed on peripheral T and B lymphocytes by bone marrow and cord blood CD34-positive cells and by mature dendritic cells. In response to its cognate chemokines, CD197/CCR7 mediates homing of leucocytes to secondary lymphoid tissues. Differential CCR7 expression can be used to distinguish naive, central memory, and effector memory T cell subsets. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17 (region 17q12). The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Caution:   Under some complex multi-color conditions, this clone may non-specifically interact with antibodies conjugated with APC-H7 or APC-Cy7, contributing to staining artifacts.  BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794), designed to minimize on non-specific fluorescent dye interactions, does not resolve this interaction with either APC-H7 or APC-Cy7.  For optimal multicolor staining results, alternatives to APC-H7 and APC-Cy7 should be evaluated.

150503 The monoclonal antibody 150503 specifically binds to the human CC chemokine receptor, CCR7, also known as CD197. CCR7 (previously known as BLR2, EBI1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3β/Exodus 3/ELC/ CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL21. CCR7 mRNA is expressed mainly in lymphoid tissues including the spleen, lymph nodes and tonsil. CD197/CCR7 is expressed on peripheral T and B lymphocytes by bone marrow and cord blood CD34-positive cells and by mature dendritic cells. In response to its cognate chemokines, CD197/CCR7 mediates homing of leucocytes to secondary lymphoid tissues. Differential CCR7 expression can be used to distinguish naive, central memory, and effector memory T cell subsets. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17 (region 17q12). The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Caution: Under some complex multi-color conditions, this clone may non-specifically interact with antibodies conjugated with APC-H7 or APC-Cy7, contributing to staining artifacts.  BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794), designed to minimize on non-specific fluorescent dye interactions, does not resolve this interaction with either APC-H7 or APC-Cy7.  For optimal multicolor staining results, alternatives to APC-H7 and APC-Cy7 should be evaluated.

Mouse IgG2a

克隆 150503 (RUO)

Flow cytometry (Routinely Tested)

5 µl

Human (QC Testing)

CD197 (CCR7)

CC chemokine receptor 7; BLR2; CMKBR7; EBI1; EVI1; MIP-3 beta receptor

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

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