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产品介绍
产品介绍
产品信息
荧光素标记
宿主
Mouse BALB/c IgG1, κ
免疫原
Human Adult Adherent Peripheral Blood Cells
简单描述
The BD FastImmune™ CD107a APC reagent is designed for the detection of degranulating T lymphocytes in activated whole blood and is intended for research use only. Applications include studies of cytotoxic CD8+ T-cell responses to viral and tumor antigens, correlation of T-cell cytolytic potential and cytokine expression, and live-cell sorting of functional antigen-specific CD8+ T cells. The CD107a detection assay can also be used as an alternative to 51Cr release assays.
Each vial supplies sufficient reagents for 50 stimulated samples and 50 unstimulated control samples. In performing the assay, 0.5 mL of whole blood is stimulated with antigen (not included) in the presence of two secretion inhibitors, monensin and brefeldin A (BFA), and CD107a antibody. For the unstimulated control, 0.5 mL of blood is treated with the secretion inhibitors and CD107a, but not antigen. Both blood samples are then stained for IFN-γ or other cytokine(s), as well as the gating markers CD3 and CD8 (not included).
商品描述
H4A3
The BD FastImmune™ CD107a APC reagent is designed for the detection of degranulating T lymphocytes in activated whole blood and is intended for research use only. Applications include studies of cytotoxic CD8+ T-cell responses to viral and tumor antigens, correlation of T-cell cytolytic potential and cytokine expression, and live-cell sorting of functional antigen-specific CD8+ T cells. The CD107a detection assay can also be used as an alternative to 51Cr release assays.
Each vial supplies sufficient reagents for 50 stimulated samples and 50 unstimulated control samples. In performing the assay, 0.5 mL of whole blood is stimulated with antigen (not included) in the presence of two secretion inhibitors, monensin and brefeldin A (BFA), and CD107a antibody. For the unstimulated control, 0.5 mL of blood is treated with the secretion inhibitors and CD107a, but not antigen. Both blood samples are then stained for IFN-γ or other cytokine(s), as well as the gating markers CD3 and CD8 (not included).
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 H4A3 (RUO (GMP))
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Flow cytometry
推荐用量
5 μL
反应种属
Human
目标/特异性
CD107a (LAMP-1)
背景
别名
LAMP1; LAMP-1; LAMPA; LGP120
数据库链接
Entrez-Gene ID
3916
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