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BD Horizon™ BV510 Mouse Anti-Human CD15S

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BD Horizon™ BV510 Mouse Anti-Human CD15S

品牌： BD Pharmingen

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产品信息

荧光素标记

BV510

抗原名称

CD15S

宿主

Mouse BALB/c IgM, κ

免疫原

Membrane proteins from human tomach adenocarcinoma tissue

简单描述

The CSLEX1 monoclonal antibody specifically binds to CD15s. This anti-Sialyl-Le[x] monoclonal antibody is specific for the α2-3 sialosylated form of lacto-N-fucopentaose III, sialyl Lex (Sle[x]). Sle[x] is expressed on granulocytes, monocytes and both normal and tumor cells of diverse origin. It has been shown to be a ligand for both endothelial leukocyte adhesion molecule-1 (ELAM-1 or E-selectin), and granule membrane protein-140 (GMP-140 or P-selectin). The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

商品描述

CSLEX1 The CSLEX1 monoclonal antibody specifically binds to CD15s. This anti-Sialyl-Le[x] monoclonal antibody is specific for the α2-3 sialosylated form of lacto-N-fucopentaose III, sialyl Lex (Sle[x]). Sle[x] is expressed on granulocytes, monocytes and both normal and tumor cells of diverse origin. It has been shown to be a ligand for both endothelial leukocyte adhesion molecule-1 (ELAM-1 or E-selectin), and granule membrane protein-140 (GMP-140 or P-selectin). The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

同种型

Mouse BALB/c IgM, κ

克隆号

克隆 CSLEX1 (RUO)

产品详情

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:BV510

激发源:Violet 405 nm

最多激发源:327 nm, 405 nm

最多发射源:512 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing)

目标/特异性

CD15s (Sialyl Lewis x)

背景

别名

Sialyl Lewis X

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(11) 1. Corral L, Singer MS, Macher BA, Rosen SD. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets. Biochem Biophys Res Commun. 1990; 172(3):1349-1356. (Biology). 2. Dransfield I, Stocks SC, Haslett C. Regulation of cell adhesion molecule expression and function associated with neutrophil apoptosis. Blood. 1995; 85(11):3264-3273. (Clone-specific: Flow cytometry). 3. Fukushima K, Hirota M, Terasaki PI. Characterization of sialosylated Lewisx as a new tumor-associated antigen. Cancer Res. 1984; 44(11):5279-5285. (Immunogen: Cytotoxicity, ELISA, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Radioimmunoassay). 4. Phillips ML, Nudelman E, Gaeta FC, et al. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990; 250(4984):1130-1132. (Biology). 5. Picker LJ, Warnock RA, Burns AR, Doerschuk CM, Berg EL, Butcher EC. The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell. 1991; 66(5):921-933. (Biology). 6. Polley MJ, Phillips ML, Wayner E, et al. CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proc Natl Acad Sci U S A. 1991; 88(14):6224-6228. (Biology). 7. Rice GE, Bevilacqua MP. An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science. 1989; 246(4935):1303-1306. (Biology). 8. Rice GE, Gimbrone MA, Bevilacqua MP. Tumor cell-endothelial interactions. Increased adhesion of human melanoma cells to activated vascular endothelium. Am J Pathol. 1988; 133(2):204-210. (Biology). 9. Walz G, Aruffo A, Kolanus W, Bevilacqua M, Seed B. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. Science. 1990; 250(4984):1132-1135. (Biology). 10. Zetter BR. The cellular basis of site-specific tumor metastasis. N Engl J Med. 1990; 322(9):605-612. (Biology). 11. Zhou Q, Moore KL, Smith DF, Varki A, McEver RP, Cummings RD. The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells. J Cell Biol. 1991; 115(2):557-564. (Clone-specific: ELISA).

参考图片

Two-parameter flow cytometric analysis of CD15s expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon™ BV510 Mouse IgM, κ Isotype Control (Cat. No. 563082; Left Panel) or BD Horizon BV510 Mouse Anti-Human CD15s antibody (Cat. No. 563529; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter dot plots show the correlated expression patterns of CD15s (or Ig Isotype control staining) versus side light-scatter characteristics for gated events with the light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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货号：

563529

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