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BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD1c

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BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD1c

品牌： BD Pharmingen

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参数

反应种属：

Human (QC Testing)

来源宿主：

Mouse IgG1, κ

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产品介绍

产品信息

耦联标记

PerCP-Cy5.5

抗原名称

CD1c

宿主

Mouse IgG1, κ

免疫原

GM-CSF- and IL-4-activated Human Monocytes

简单描述

The F10/21A3 monoclonal antibody specifically binds to CD1c. The CD1 family of transmembrane glycoproteins are structurally related to the classical major histocompatibility complex (MHC) proteins. CD1c is a type I transmembrane glycoprotein that forms heterodimers with beta-2-microglobulin. CD1c presents lipids and glycolipids of self or microbial origin to T cells. CD1c is expressed by Langerhans cells, dendritic cells, monocytes, cortical thymocytes, T cells, and some B cells.

商品描述

F10/21A3 The F10/21A3 monoclonal antibody specifically binds to CD1c. The CD1 family of transmembrane glycoproteins are structurally related to the classical major histocompatibility complex (MHC) proteins. CD1c is a type I transmembrane glycoprotein that forms heterodimers with beta-2-microglobulin. CD1c presents lipids and glycolipids of self or microbial origin to T cells. CD1c is expressed by Langerhans cells, dendritic cells, monocytes, cortical thymocytes, T cells, and some B cells.

同种型

Mouse IgG1, κ

克隆号

克隆 F10/21A3 (RUO)

产品详情

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PerCP-Cy5.5

激发源:Blue 488 nm

最多激发源:482 nm

最多发射源:676 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing)

目标/特异性

CD1c

背景

别名

CD1; R7; M241; BDCA1

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(4) 1. Delia D, Cattoretti G, Polli N, et al. CD1c but neither CD1a nor CD1b molecules are expressed on normal, activated, and malignant human B cells: identification of a new B-cell subset. Blood. 1988; 72(1):241-247. (Biology). 2. Grant EP, Degano M, Rosat JP, et al. Molecular recognition of lipid antigens by T cell receptors. J Exp Med. 1999; 189(1):195-205. (Clone-specific: Blocking, Functional assay, Inhibition). 3. Melian A, Geng YJ, Sukhova GK, Libby P, Porcelli SA. CD1 expression in human atherosclerosis. A potential mechanism for T cell activation by foam cells. Am J Pathol. 1999; 155(3):775-786. (Immunogen: Flow cytometry). 4. Moody DB, Ulrichs T, Mühlecker W, et al. CD1c-mediated T-cell recognition of isoprenoid glycolipids in Mycobacterium tuberculosis infection. Nature. 2000; 404(6780):884-888. (Clone-specific: Blocking, Functional assay, Inhibition).

参考图片

Two-color flow cytometric analysis of CD1c expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 555412/560994) and either PerCP-Cy™5.5 Mouse IgG1, κ Isotype Control (Cat. No. 550795; Left Panel) or PerCP-Cy™5.5 Mouse Anti-Human CD1c (Cat. No. 565423/565424; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric contour plots showing the correlated expression of CD1c (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

Two-color flow cytometric analysis of CD1c expression on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 555412/560994) and either PerCP-Cy™5.5 Mouse IgG1, κ Isotype Control (Cat. No. 550795; Left Panel) or PerCP-Cy™5.5 Mouse Anti-Human CD1c (Cat. No. 565423/565424; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric contour plots showing the correlated expression of CD1c (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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货号：

565424

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