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产品介绍
产品介绍
产品信息
荧光素标记
宿主
Mouse BALB/c IgG1, κ
免疫原
Human Phytohemagglutinin-activated T Cells
商品描述
2A3
The CD25 antibody, clone 2A3. is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with PHA-activated human T lymphocytes.
The CD25 antibody recognizes a 55-kilodalton (kDa) type I integral membrane glycoprotein. The CD25 antigen is also known as the human low-affinity interleukin-2 receptor α chain (IL-2Rα) and the Tac antigen.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 2A3 (RUO (GMP))
浓度
12.5 μg/mL
产品详情
PE-Cy7
PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-Cy7
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
781 nm
应用
实验应用
Flow cytometry
推荐用量
5 μL
反应种属
Human
目标/特异性
CD25 (IL-2 Receptor α)
背景
别名
IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
制备和贮存
存储溶液
Phosphate buffered saline with gelatin and 0.1% sodium azide.
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