CD274 (B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1)

Human

Mouse

Recombinant protein fragment corresponding to aa24-153 of human CD274 expressed in E. coli.

Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested.

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

IgG2b

7D2A10

Monoclonal

Purified

Supplied as a liquid in PBS, 0.05% sodium azide

E FC WB

Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.

Mouse

Hu

Recognizes human CD274.

29126