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PD-L1 (E1L3N®) XP® Rabbit mAb

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PD-L1 (E1L3N®) XP® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

PD-L1

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
IHC Leica Bond	1:200 - 1:800
Immunohistochemistry (Paraffin)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Rabbit IgG

分子量

40-50

研究领域

癌症,免疫学和肿瘤学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

PD-L1 (E1L3N) XP Rabbit mAb recognizes endogenous levels of total PD-L1 protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

别名

PD-L1,PDL1,CD274,B7-H1,B7H1,hPD-L1,PD-L1(B7-H1),PDCD1L1,PDCD1LG1,Programmed cell death 1 ligand 1,PDCD1 ligand 1

背景

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). 1.Dong, H. et al. (1999) Nat Med 5, 1365-9. 2.Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34. 3.Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16. 4.Dong, H. et al. (2002) Nat Med 8, 793-800. 5.Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5. 6.Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64. 7.Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37. 8.Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41. 9.Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73. 10.Wimberly, H. et al. (2014) Cancer Immunol Res , .

研究领域

癌症,免疫学和肿瘤学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #85164.

数据库链接

Entrez-Gene ID

29126

UniProt ID

Q9NZQ7

参考图片

Flow cytometric analysis of Ramos cells (blue, negative) and Sup-M2 cells (green, positive) using PD-L1 (E1L3N®) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from KARPAS-299, SUP-M2, and PC-3 cells using PD-L1 (E1L3N®) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from A549 cells, IFN-γ treated (100 ng/mL, 48 hr; +) or untreated (-), using PD-L1 (E1L3N®) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of PD-L1 protein from KARPAS-299 extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PD-L1 (E1L3N®) XP® Rabbit mAb. Western blot analysis was performed using PD-L1 (405.9A11) Mouse mAb #29122. Anti-mouse IgG, HRP-linked Antibody #7076 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using PD-L1 (E1L3N®) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 (E1L3N®) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human placenta using PD-L1 (E1L3N®) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded Karpas-299 (left) or PC-3 (right) cell pellets using PD-L1 (E1L3N®) XP® Rabbit mAb.

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-L1 (E1L3N®) XP® rabbit mAb (red), CD8α (C8/144B) mouse mAb #70306 (magenta), B7-H4 (D1M8I) XP® rabbit mAb #14572 (green), FoxP3 (D2W8E™) rabbit mAb #98377 (yellow), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-L1 (E1L3N®) XP® rabbit mAb (red), PD-L2 (D7U8C™) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M™) XP® rabbit mAb #93668 (green), IDO (D5J4E™) rabbit mAb #86630 (yellow), CD68 (D4B9C) XP® rabbit mAb #76437 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma using PD-L1 (E1L3N®) XP® rabbit mAb (red), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

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货号：

13684T

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