Ms CD274 (PD-L1) Alexa 647 10F.9G2 25ug

CD274 (PD-L1)

Rat LEW, also known as Lewis IgG2b, κ

Mouse PD-L1

The 10F.9G2 monoclonal antibody specifically recognizes Programmed cell death 1 ligand 1 (PDCD1 ligand 1) which is also known as Programmed death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), or CD274. CD274 (PD-L1) is a 43-kDa type I transmembrane glycoprotein that is encoded by Cd274 which belongs to the B7 family within the Ig superfamily. CD274 (PD-L1) is expressed at low levels on resting peripheral T and B lymphocytes, monocytes, macrophages, dendritic cells (DC), and NK cells and undergoes upregulated expression upon cellular activation. PD-L1 (CD274) is also widely expressed on nonhematopoietic cell types including epithelial cells, endothelial cells, placental trophoblasts, and tumor cells. CD274 (PD-L1) and CD273 (PD-L2) serve as ligands for the CD279 (Programmed cell death protein 1/PD-1) inhibitory receptor. CD274 (PD-L1)-mediated signaling through CD279 (PD-1) regulates T cell responses in lymphoid and nonlymphoid tissues that are important for ensuring protective immunity while maintaining peripheral tolerance. This immune signaling checkpoint may suppress antitumor immune responses and prevent tumor rejection. The 10F.9G2 antibody reportedly blocks CD274 (PD-L1) binding to CD279 (PD-1) and can enhance the proliferation and effector responses of activated T cells including cytokine production and cytolytic activity.

10F.9G2 The 10F.9G2 monoclonal antibody specifically recognizes Programmed cell death 1 ligand 1 (PDCD1 ligand 1) which is also known as Programmed death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), or CD274. CD274 (PD-L1) is a 43-kDa type I transmembrane glycoprotein that is encoded by Cd274 which belongs to the B7 family within the Ig superfamily. CD274 (PD-L1) is expressed at low levels on resting peripheral T and B lymphocytes, monocytes, macrophages, dendritic cells (DC), and NK cells and undergoes upregulated expression upon cellular activation. PD-L1 (CD274) is also widely expressed on nonhematopoietic cell types including epithelial cells, endothelial cells, placental trophoblasts, and tumor cells. CD274 (PD-L1) and CD273 (PD-L2) serve as ligands for the CD279 (Programmed cell death protein 1/PD-1) inhibitory receptor. CD274 (PD-L1)-mediated signaling through CD279 (PD-1) regulates T cell responses in lymphoid and nonlymphoid tissues that are important for ensuring protective immunity while maintaining peripheral tolerance. This immune signaling checkpoint may suppress antitumor immune responses and prevent tumor rejection. The 10F.9G2 antibody reportedly blocks CD274 (PD-L1) binding to CD279 (PD-1) and can enhance the proliferation and effector responses of activated T cells including cytokine production and cytolytic activity.

Rat LEW, also known as Lewis IgG2b, κ

克隆 10F.9G2 (RUO)

0.2 mg/ml

Flow cytometry (Routinely Tested)

Mouse (QC Testing)

CD274 (PD-L1)

Cd274; Pdl1; Pdcd1l1; Pdcd1lg1; B7 homolog 1; B7h1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(6) 1. Eppihimer MJ, Gunn J, Freeman GJ, et al. Expression and regulation of the PD-L1 immunoinhibitory molecule on microvascular endothelial cells. Microcirculation. 2002; 9(2):133-145. (Immunogen: ELISA, Flow cytometry, Immunohistochemistry, Radioimmunoassay). 2. Freeman GJ, Long AJ, Iwai Y, et al. Engagement of PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000; 192:1027-1034. (Biology). 3. Iraolagoitia XL, Spallanzani RG, Torres NI, et al. NK Cells Restrain Spontaneous Antitumor CD8+ T Cell Priming through PD-1/PD-L1 Interactions with Dendritic Cells.. J Immunol. 2016; 197(3):953-61. (Clone-specific: Flow cytometry). 4. Paterson AM, Brown KE, Keir ME, et al. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo.. J Immunol. 2011; 187(3):1097-105. (Clone-specific: Blocking). 5. Rodig N, Ryan T, Allen JA, et al. Endothelial expression of PD-L1 and PD-L2 down-regulates CD8+ T cell activation and cytolysis.. Eur J Immunol. 2003; 33(11):3117-26. (Clone-specific: Blocking, Flow cytometry, Functional assay). 6. Sharpe AH, Wherry EJ, Ahmed R, Freeman GJ. The function of programmed cell death 1 and its ligands in regulating autoimmunity and infection.. Nat Immunol. 2007; 8(3):239-45. (Biology).