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WB result of PD-L1 Rabbit mAb Primary antibody: PD-L1 Rabbit mAb at 1/1000 dilution Lane 1: Untreated A549 whole cell lysate 20 µg Lane 2: A549 treated with IFNγ (100 ng/ml, 48 hr) whole cell lysate 20 µg Negative control: Untreated A549 whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 33 kDa Observed MW: 40~50 kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PD-L1 antibody was used at 1/500 dilution, Secondary antibody: #abs20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PD-L1 antibody was used at 1/500 dilution, Secondary antibody: #abs20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical carcinoma. Anti-PD-L1 antibody was used at 1/500 dilution, Secondary antibody: #abs20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-PD-L1 antibody was used at 1/500 dilution, Secondary antibody: #abs20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.