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BD Pharmingen™ Alexa Fluor® 700 Hamster anti-Mouse CD3e

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BD Pharmingen™ Alexa Fluor® 700 Hamster anti-Mouse CD3e

品牌： BD Pharmingen

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产品介绍

产品信息

抗原名称

CD3e

宿主

Syrian Hamster IgG2, κ

免疫原

Mouse T-cell receptor

简单描述

The 500A2 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on mouse thymocytes, mature T lymphocytes, and NKT cells. Plate-bound and soluble forms of the 500A2 antibody can activate T cells in vitro . Activation of a mouse T-cell clone by the 500A2 antibody can be blocked by Fab fragments of the GK1.5 anti-CD4 antibody. This suggests that the 500A2 antibody may bind an epitope on CD3e close to a site at which CD4 associates with the T-cell receptor. The 500A2 antibody reportedly does not to crossreact with rat leukocytes.

商品描述

500A2 The 500A2 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on mouse thymocytes, mature T lymphocytes, and NKT cells. Plate-bound and soluble forms of the 500A2 antibody can activate T cells in vitro . Activation of a mouse T-cell clone by the 500A2 antibody can be blocked by Fab fragments of the GK1.5 anti-CD4 antibody. This suggests that the 500A2 antibody may bind an epitope on CD3e close to a site at which CD4 associates with the T-cell receptor. The 500A2 antibody reportedly does not to crossreact with rat leukocytes.

同种型

Syrian Hamster IgG2, κ

克隆号

克隆 500A2 (RUO)

浓度

0.2 mg/ml

产品详情

Alexa Fluor™ 700

Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

CD3e

背景

别名

CD3; CD3 epsilon; Cd3e; CD3ε; T3e

制备和贮存

存储溶液

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

文献

研发参考(5) 1. Allison JP, Havran WL, Poenie M, et al. Expression and function of CD3 on murine thymocytes. In: Kappler J, Davis M, ed. The T-Cell Receptor, UCLA Symposia, 73rd Edition. Los Angeles: 1988:33-45. 2. Havran WL, Poenie M, Kimura J, Tsien R, Weiss A, Allison JP. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987; 330(6144):170-173. (Clone-specific). 3. Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Methodology: Flow cytometry). 4. Ortaldo JR, Winkler-Pickett R, Mason AT, Mason LH. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol. 1998; 160(1):1158-1165. (Clone-specific). 5. Portoles P, Rojo J, Golby A, et al . Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989; 142(12):4169-4175. (Clone-specific).

数据库链接

Entrez-Gene ID

12501

参考图片

CD3e expression in spleen. C57BL/6 splenocytes were simultaneously stained with PE rat anti-mouse CD4 mAb RM4-5 (Cat. no. 553048 or 553049), PE rat anti-mouse CD8a mAb 53-6.7 (Cat. no. 553032 or 553033), and either Alexa Fluor® 700 Hamster IgG2, λ Isotype Control (Cat. no. 557985, left panel) or Alexa Fluor® 700 Hamster anti-Mouse CD3e (right panel). Flow cytometry was performed on a BD™ LSR II flow cytometry system.

CD3e expression in thymus. C57BL/6 thymocytes were stained with either the same isotype control (dashed line) or Alexa Fluor® 700 Hamster anti-Mouse CD3e (solid line). Flow cytometry was performed on a BD™ LSR II flow cytometry system

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货号：

557984

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100ug

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