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品牌: BD Pharmingen









反应种属:
Mouse (QC Testing)
来源宿主:
Armenian Hamster IgG1, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
CD3e

宿主
Armenian Hamster IgG1, κ

免疫原
H-2Kb specific cytotoxic T lymphocyte clone BM10-37

商品描述
145-2C11

同种型
Armenian Hamster IgG1, κ

克隆号
克隆 145-2C11 (RUO)

浓度
0.2 mg/ml

产品详情
BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

BV711
Violet 405 nm
407 nm
713 nm
应用
实验应用
Flow cytometry (Routinely Tested)

反应种属
Mouse (QC Testing)

目标/特异性
CD3e

背景
别名
CD3; CD3 epsilon; Cd3e; CD3ε; T3e

制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
12501

参考图片
Two-color flow cytometric analysis of CD3e expressed on mouse splenocytes. BALB/c splenic leucocytes were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093) antibodies and either BD Horizon™ BV711 Armenian Hamster IgG1, κ Isotype Control (Cat. No. 563128; Left Panel) or BD Horizon™ BV711 Hamster Anti-Mouse CD3e antibody (Cat. No. 563123; Right Panel). The two-color flow cytometric dot plots show CD3 (or Ig Isotype Control staining) versus CD4 and CD8 derived from events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.
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