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BD Pharmingen™ Purifed NA/LE Mouse Anti-Human CD3

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BD Pharmingen™ Purifed NA/LE Mouse Anti-Human CD3

品牌： BD Pharmingen

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产品介绍

产品信息

抗原名称

CD3

宿主

Mouse BALB/c x A/J, also known as CAF1 IgG2a, κ

免疫原

Sheep Erythrocyte Rosette-purified Human T Cells

简单描述

The OKT3 monoclonal antibody specifically recognizes the CD3 epsilon subunit (CD3e/CD3ε) of the CD3 complex which consists of four transmembrane proteins (γ, δ, ε, ζ) that are associated with the T cell antigen receptor (TCR) to form the CD3/TCR complex. The CD3 complex associates with either TCR αβ or TCR γδ heterodimers that are alternatively expressed by some thymocytes, T cells or NKT cells. The CD3 complex is required for the cell surface expression and signal-transducing functions of the TCR. The CD3 complex is expressed by ~60-85% thymocytes and by all peripheral mature T cells. CD3e is also known as T3E or TCRE. CD3e is a ~20 kDa unglycosylated type I transmembrane protein that is encoded by CD3E which belongs to the immunoglobulin superfamily (IgSF). CD3e has an Ig-like extracellular domain (ECD) and an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The OKT3 antibody can reportedly fix complement, stimulate T cell proliferation and cytokine production, and block the binding of other human CD3e-specific antibodies including UCHT1 and SK7.

商品描述

OKT3 The OKT3 monoclonal antibody specifically recognizes the CD3 epsilon subunit (CD3e/CD3ε) of the CD3 complex which consists of four transmembrane proteins (γ, δ, ε, ζ) that are associated with the T cell antigen receptor (TCR) to form the CD3/TCR complex. The CD3 complex associates with either TCR αβ or TCR γδ heterodimers that are alternatively expressed by some thymocytes, T cells or NKT cells. The CD3 complex is required for the cell surface expression and signal-transducing functions of the TCR. The CD3 complex is expressed by ~60-85% thymocytes and by all peripheral mature T cells. CD3e is also known as T3E or TCRE. CD3e is a ~20 kDa unglycosylated type I transmembrane protein that is encoded by CD3E which belongs to the immunoglobulin superfamily (IgSF). CD3e has an Ig-like extracellular domain (ECD) and an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The OKT3 antibody can reportedly fix complement, stimulate T cell proliferation and cytokine production, and block the binding of other human CD3e-specific antibodies including UCHT1 and SK7.

同种型

Mouse BALB/c x A/J, also known as CAF1 IgG2a, κ

克隆号

克隆 OKT3 (RUO)

浓度

1.0 mg/ml

产品详情

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

应用

实验应用

Activation, Flow cytometry (Tested During Development)

反应种属

Human (QC Testing)

目标/特异性

CD3

背景

别名

CD3E; CD3e; T-cell surface antigen T3/Leu-4 epsilon; T3E; TCRE

制备和贮存

存储溶液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

保存方式

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

文献

研发参考(12) 1. Burns GF, Boyd AW, Beverley PC. Two monoclonal anti-human T lymphocyte antibodies have similar biologic effects and recognize the same cell surface antigen. J Immunol. 1982; 129(4):1451-1457. (Clone-specific: Blocking, Functional assay, Immunoprecipitation, Radioimmunoassay). 2. Emmrich F. Selective stimulation of human CD4 and CD8 T-cells by crosslining the T-cell receptor with subset-specific differentiation antigens. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:203-206. 3. Ernst DN, Shih CC. CD3 complex. J Biol Regul Homeost Agents. 2000; 14(3):226-229. (Biology). 4. Horibe K, Knowles RW, Naito K, Morishima Y, Dupont B. Analysis of T lymphocyte antibody specificities: Comparison of serology with immunoprecipitation patterns. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies. Berlin New York: Springer-Verlag; 1984:212-224. 5. Kung P, Goldstein G, Reinherz EL, Schlossman SF. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979; 206(4416):347-349. (Immunogen: Cytotoxicity, Flow cytometry, Radioimmunoassay). 6. Kurrle R, Seyfert W, Trautwein A, Seiler FR. T cell activation by CD3 antibodies. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:137-146. 7. Li B, Wang H, Dai J, et al. Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005; 116(4):487-498. (Clone-specific: Blocking, Flow cytometry). 8. Semnani R, Nutman TB, Corrado G, Hochman P, Shaw S, Van Seventer GA. Costimulation mediated by purified ICAM-1 and LFA-3 regulates differential stimulation and cytokine secretion of human 'naive' and 'memory' CD4+ T cells. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens. Oxford: Oxford University Press; 1995:1488-1491. 9. Touraine JL, Favrot MC, Ansary ME, Cordier G, de bouteiller O. Phenotype of prothymocytes from human bone marrow determined by monoclonal antibodies: Modification induced by thymic factots. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies. Berlin New York: Springer-Verlag; 1984:298-311. 10. Tunnacliffe A, Olsson C, Traunecker A, Krissansen GW, Karjalainen K, de la Hera A. The majority of CD3 epitopes are conferred by the epsilon chain. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:295-296. 11. Van Wauwe JP, De Mey JR, Goossens JG. OKT3: a monoclonal anti-human T lymphocyte antibody with potent mitogenic properties. J Immunol. 1980; 124:2708-2713. (Clone-specific: Functional assay). 12. Van Wauwe JP, Goossens JG, Beverley PC. Human T lymphocyte activation by monoclonal antibodies; OKT3, but not UCHT1, triggers mitogenesis via an interleukin 2-dependent mechanism. J Immunol. 1984; 133(1):129-132. (Clone-specific: Functional assay).

参考图片

CD3 expression and function Left Panel -Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes (PBL). Peripheral blood was incubated with either Purified Mouse IgG2a κ Isotype Control (Cat. No. 553454; dashed line histogram) or Purified NA/LE Mouse Anti-Human CD3 antibody (Cat. No. 566685; solid line histogram). The cells were washed and stained with PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histogram showing CD3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Multicolor flow cytometric analysis of CD25 expression on unstimulated and Anti-CD3 plus Anti-CD28 antibody-stimulated human PBL. Human peripheral blood mononuclear cells (PBMC) were not stimulated [Unstimulated: Bottom red-colored histograms] or were stimulated (72 h) with either soluble Purified NA/LE Mouse Anti-Human CD3 antibody (0.5 μg OKT-3 Ab/ml) plus soluble Purified NA/LE Mouse Anti-Human CD28 antibody (5 μg Ab/ml; Cat. No. 555725) [Midlevel orange histograms; sol CD3/sol CD28] or plate-bound Mouse Anti-Human CD3 antibody (5 μg OKT-3 Ab/ml overnight coating) plus Purified NA/LE Mouse Anti-Human CD28 antibody (5 μg Ab/ml) [Top blue histograms; pb CD3/sol CD28] as indicated. The cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD25 (Cat. No. 562442/564033), APC-H7 Mouse Anti Human CD4 (Cat. No. 560158/560251) and BD Horizon™ BUV395 Mouse Anti-Human CD8 (Cat. No. 563795/ 563796) antibodies. The fluorescence histograms showing CD25 expression were derived from either CD4+ (Left Histograms) or CD8+ (Right Histograms) gated events with the light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

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货号：

566685

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