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Hu CD3 RB705 UCHT1 100Tst
品牌： BD Pharmingen
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咨询反应种属：
Human (QC Testing)
实验应用：
Flow cytometry (Routinely Tested)
产品介绍
产品使用步骤推荐 产品介绍
产品信息
荧光素标记
RB705()
免疫原
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
简单描述
The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.
商品描述
UCHT1
The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 UCHT1 (also known as UCHT-1; UCHT 1) (RUO)
产品详情
RB705
The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.
RB705
498 nm
707 nm
BD化合物表
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应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
5 µl/test
反应种属
Human (QC Testing)
目标/特异性
CD3
背景
别名
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
916
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