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宿主
亚美尼亚仓鼠
克隆号
145-2C11 f(ab')2 Fragments
Product Details
The anti-mouse CD3 F(ab’)2 fragment is the antigen binding F(ab’)2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody F(ab’)2 fragment reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The complete 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo activation of resting T cells results in cytokine release and toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor-bearing cells. To avoid these complications non-Fc receptor binding F(ab’)2 fragments of the 145-2C11 antibody are commonly used. This non-FcR-binding anti-CD3 induces apoptosis of Ag-activated T cells in vivo by allowing durable expression of the TCR and sustained signaling. Foxp3+ Tregs have been shown to be resistant to CD3 antibody-mediated depletion.Specifications
| Isotype | Armenian Hamster IgG1 |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb hamster IgG f(ab')2 fragments |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse BM10-37 cytotoxic T cells |
| Reported Applications | in vivo T cell depletion |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 μM filtered |
| Production | Pepsin Digest |
| Purification | Protein A |
| RRID | AB_2687679 |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
组成成分
跨膜细胞表面蛋白抗体，磷酸盐缓冲液
背景
研究领域
Immunology
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