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hCD3 MAb (Cl UCHT1) (25 ug)
hCD3 MAb (Cl UCHT1) (25 ug)
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hCD3 MAb (Cl UCHT1) (25 ug)

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品牌： R&D Systems
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来源宿主：
Mouse IgG1
实验应用：
Flow Cytometry(0.25 µg/106 cells)
Immunocytochemistry(8-25 µg/mL)
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
CD3 epsilon， CD3e antigen， CD3e antigen, epsilon polypeptide (TiT3 complex)， CD3e molecule, epsilon
宿主
Mouse IgG1
免疫原
Human thymocytes (1)
克隆号
UCHT1

Scientific Data

Immunocytochemistry CD3e antibody in Human PBMCs by Immunocytochemistry (ICC).View Larger

CD3 epsilon in Human PBMCs. CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot View Larger

Detection of Human CD3 epsilon by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of CD3e antibody in Human Lymphocytes antibody by Flow Cytometry.View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse CD3 epsilon by Flow CytometryView Larger

Detection of Mouse CD3 epsilon by Flow Cytometry The representative figures for T cell subsets counted by flow cytometry from tumors in B16-F10-bearing mice.The cell counts for CD3+ (A, B and C), CD3+CD4+ (D, E and F), CD3+CD8+ (D, E and F), CD4+CD25+CD127low/- (G, H and I) and CD8+IFN-gamma + (J, K and L) T lymphocytes from mouse tumor were determined by flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human CD3 epsilon Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
 
CyTOF-reported
Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
T Cell Stimulation
This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Flow Cytometry(0.25 µg/106 cells)
Immunocytochemistry(8-25 µg/mL)
背景
别名
CD3 epsilon,CD3e antigen,CD3e antigen, epsilon polypeptide (TiT3 complex),CD3e molecule, epsilon (CD3-TCR complex),CD3e,CD3-epsilon,FLJ18683,T3E,T-cell antigen receptor complex, epsilon subunit of T3,T-cell surface antigen T3/Leu-4 epsilon chain,T-cell surface glycoprotein CD3 epsilon chain,TCRE
背景

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
研究领域
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

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货号：
MAB100-SP
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