BD IMag™ Human CD4 T Lymphocyte Enrichment Set-DM

Enrichment of CD4 T lymphocytes from human blood. PBMC were labeled with the BD IMag™ Human CD4 T Lymphocyte Enrichment Set - DM (Cat. No. 557939) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying protocol. Cells were stained with APC Mouse Anti-Human CD4 (Cat. No. 555349) to detect CD4 T lymphocytes and a mixture of PE Mouse Anti-Human CD8 (555367), CD11b/Mac-1  (Cat. No. 555388), CD16 (Cat. No. 555407), CD19 (Cat. No. 555413), CD36  (Cat. No. 555455), CD56 (Cat. No. 555516), CD123 (Cat. No. 554529), CD235a (Cat. No. 555570), and γδ TCR (Cat. No. 555717) monoclonal antibodies to detect non-CD4 T leukocytes and erythrocytes.  Dead cells were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Refer to the Enrichment Flow Chart to identify the cell populations represented. The percentage of CD4 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in top right panel. This additional separation step can result in up to 2-6% increased purity with less than a 5% decrease in recovery. Far right panel shows the positive fraction.