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Rabbit anti-CD4 Polyclonal Antibody
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对比
咨询Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
>95%
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
>95%
参考图片
Sample:
HL60 Cell Lysate at 40 ug
Primary: Anti- CD4 (abs120100) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
U937 Cell Lysate at 40 ug
Primary: Anti- CD4 (abs120100) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- CD4 (abs120100) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
Sample:
Lymph node(Mouse) Lysate at 40 ug
Primary: Anti- CD4 (abs120100) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55kD
Blank control (Black line): Molt4 (Black). Primary antibody: Rabbit Anti-CD4 antibody Dilution: 3μg /10^6 cells; Isotype Control antibody: Rabbit IgG-PE.ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary antibody: Rabbit Anti-CD4 antibody,Dilution: 1μg /10^6 cells;
Isotype Control antibody: Rabbit IgG .
Secondary antibody: Goat anti-rabbit IgG-FITC,dilution: 1μg /test.