Product Details

The MIAP410 monoclonal antibody reacts with mouse CD47 otherwise known as integrin-associated protein (IAP). CD47 is an approximately 50 kDa glycosylated five transmembrane protein that is ubiquitously expressed by both hematopoietic cells such as T and B lymphocytes, monocytes, platelets and erythrocytes and non-hematopoietic cells. CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is a receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis. CD47 Is has been found to be overexpressed in many different tumor cells. Because of this, anti-CD47 monoclonal antibodies have been proposed and studied as a therapeutic treatment for human cancers. The MIAP410 antibody has been shown to neutralize CD47 in vivo and in vitro.

Specifications

Isotype	Mouse IgG1, κ
Recommended Isotype Control(s)	InVivoPlus mouse IgG1 isotype control, unknown specificity
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen	purified human placental CD47
Reported Applications	in vivo CD47 blockade in vitro CD47 blocking Immunofluorescence
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin*	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation*	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein G
RRID	AB_2687806
Molecular Weight	150 kDa
Murine Pathogen Tests*	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

* Additional quality control measures for our InVivoPlus™ products include advanced binding validation, murine pathogen screening, protein aggregation screening, and ultra-low endotoxin levels. The superior quality of our InVivoPlus™ products will meet and exceed the strict demands and rigorous standards required for in vivo research. Learn more about the InVivoPlus™ difference here.

组成成分

整合素相关蛋白抗体，磷酸盐缓冲液

应用

反应种属

mouse/human/rat

背景

研究领域

Apoptosis, Cancer Biology, Cell Migration & Adhesion, Immunology, Neuroscience

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货号：

BP0283-5MG

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