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BD Horizon™ PE-CF594 Mouse Anti-Human CD70
品牌： BD Pharmingen
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咨询反应种属：
Human (QC Testing)
来源宿主：
Mouse IgG3, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
CD70
宿主
Mouse IgG3, κ
免疫原
Human L428 Cell Line
简单描述
The Ki-24 monoclonal antibody specifically binds to human CD70. CD70 is a type II transmembrane glycoprotein and member of the TNF Superfamily. CD70 is also known as Tumor necrosis factor ligand superfamily member 7 (TNFSF7), CD27 ligand (CD27-L, CD27L, CD27LG), and KI-24 antigen. The CD70 antigen immunoprecipitates as five bands (50, 70, 90, 100 and 160 kDa) under non-reducing conditions. CD70 is strongly expressed on Reed-Sternberg cells, some activated T or B cells and Epstein Barr Virus (EBV)-positive lymphoblastoid cell lines. CD70 plays roles in the activation, proliferation and differentiation of B cells and T cells including the enhanced production of cytotoxic T cells.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
商品描述
Ki-24
The Ki-24 monoclonal antibody specifically binds to human CD70. CD70 is a type II transmembrane glycoprotein and member of the TNF Superfamily. CD70 is also known as Tumor necrosis factor ligand superfamily member 7 (TNFSF7), CD27 ligand (CD27-L, CD27L, CD27LG), and KI-24 antigen. The CD70 antigen immunoprecipitates as five bands (50, 70, 90, 100 and 160 kDa) under non-reducing conditions. CD70 is strongly expressed on Reed-Sternberg cells, some activated T or B cells and Epstein Barr Virus (EBV)-positive lymphoblastoid cell lines. CD70 plays roles in the activation, proliferation and differentiation of B cells and T cells including the enhanced production of cytotoxic T cells.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
同种型
Mouse IgG3, κ
克隆号
克隆 Ki-24 (RUO)
产品详情
PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
5 µl
反应种属
Human (QC Testing)
目标/特异性
CD70
背景
别名
CDw70; CD27 ligand; CD27-L; CD27L; CD27LG; Ki-24 antigen; TNFSF7
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Bowman MR, Crimmins MA, Yetz-Aldape J, Kriz R, Kelleher K, Herrmann S. The cloning of CD70 and its identification as the ligand for CD27. J Immunol. 1994; 152(4):1756-1761. (Biology).
2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
4. Stein H, Ferszt A, Dallenbach F, et al. CDw70 mAb A109 (Ki-24): expression by reactive and neoplastic lymphoid cells. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:449-451.
5. Stein H, Gerdes J, Lemke H, Mason DY. Evidence of Sternberg-Reed cells being derived from activated lymphocytes. Haematol Blood Transfus. 1985; 29:441-444. (Clone-specific: Immunocytochemistry (cytospins)).
6. Stein H, Gerdes J, Schwab U, et al. Evidence for the detection of the normal counterpart of Hodgkin and Sternberg-Reed cells.. Hematol Oncol. 1(1):21-9. (Clone-specific).
7. Stein H, Gerdes J, Schwarting R, Froese P, Lemke H. Three new lymphoid activation antigens. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:574.
8. Stein H, Schwarting R, Niedobitek G, Dallenbach F. Cluster report: CDw70. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:446-449.
参考图片
Flow cytometric analysis of CD70 expressed on U266 human plasma cell line. Human U266 cells were stained with either BD Horizon™ PE-CF594 Mouse Anti-Human CD70 antibody (Cat. No. 562484, solid line histogram) or a BD Horizon™ PE-CF594 mIgG3, κ Isotype Control (Cat. No. 562482; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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