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品牌： BD Pharmingen
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对比
咨询反应种属：
Mouse (QC Testing)
来源宿主：
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
IgA
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled mouse IgA
简单描述
The C10-3 antibody reacts specifically with mouse IgA of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes.
商品描述
C10-3
The C10-3 antibody reacts specifically with mouse IgA of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 C10-3 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgA
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Wrammert J, Källberg E, Agace WW, Leanderson T. Ly6C expression differentiates plasma cells from other B cell subsets in mice. Eur J Immunol. 2002; 32(1):97-103. (Clone-specific: Flow cytometry, Immunofluorescence).
参考图片
Detection of intracellular mouse IgA in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained 5according to the method described below using FITCconjugated C10-3 mAb (solid line, Cat. no. 559354) or the matched isotype control, FITCconjugated R3-34 mAb (dotted line, Cat. no. 554684). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
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